Cysteine�rich with EGF�like domain protein 1 (CRELD1) is an ~50kD transmembrane glycoprotein that contains a 333aa extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15aa (1). Within the large ECD, mouse and human CRELD1 share 92%aa sequence identity. CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro�apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha�1 receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4).
Source:
Recombinant corresponding to aa30�362 from mouse CRELD1 at C-terminal, expressed in NS0 cells (Q91XD7).
Molecular Weight:
~37.1kD
Biological Activity:
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.3-1.5ug/ml.
Endotoxin:
<0.10 EU per 1ug of protein by the LAL method
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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