Crystal 50x TAE buffer is a Tris-Acetate-EDTA electrophoresis running buffer made from reagents with the highest purity and delivered in convenient ready-to-use sealed pouches. Each pouch contains a pre-determined amount of components, providing increased buffer precision. There is no need to spend time in the laboratory weighing and handling the loose components for the buffers. Simply dissolve the contents of each the pouch in deionized water to the recommended volume and the buffer is ready for use without any further preparation. Crystal 50x TAE Buffer is recommended for large fragments (above 1500bp) and complex mixtures of large genomic DNA. TAE has a lower buffering capacity than TBE, however linear dsDNA tends to migrate faster in TAE than in TBE. Crystal 50xTAE Buffer is also used in non-denaturing RNA agarose gel electrophoresis. Southern blots are generally derived from gels prepared and run in TAE buffers. Bioline Crystal buffers undergo extensive and stringent QC assays and the guaranteed batch-to-batch reproducibility provides consistent results. One pouch of Crystal 50x TAE buffer dissolved in deionized water and made up to 1000ml yields a 10x stock solution containing 2.0M Tris-Acetate, 0.05M EDTA, pH 8.3 at 25°C.
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