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Products Cyclic GMP EIA Kit (without Acetic Anhydride)
| Catalogue number: | 581022 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Cyclic Nucleotides |
| Shipping temp: | -20 |
| Storage temp: | -20 |
| Additional info: | cGMP is a key intracellular second messenger molecule which transduces cellular signaling events in response to a variety of hormones, autacoids and drugs. cGMP is synthesized from GTP by both membrane-bound and soluble guanlyate cyclase enzymes. The relative abundance of cGMP within a given cell can serve as a marker for activation by agonists acting through particulate guanylate cyclase at the cell surface, or intracellular activation of soluble guanylate cyclase by nitric oxide. Cayman's cGMP Assay is a competitive EIA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma or urine. The EIA typically displays an IC50 value (50% B/B0) of approximately 5 pmol/ml and a detection limit (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2 |
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