The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure.
Sample Type:
Cell culture (adherent and suspension), fresh and frozen tissues.
Intended Use:
The Cytosol/Particulate Separation Kit provides an easy and convenient system for a rapid separation of cytosol and particulate fractions. Assaying cellular distributions of small moleculars or proteins, and other signal transduction pathways.
Test Principle:
The new Cytosol/Particulate Rapid Separation BioAssay(TM) Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
Kit Components:
Cell Suspension buffer, 1x2ml
Cytosol Releasing Buffer, 1x2ml
Oil Layer, 1x25ml
Particulate Layer, 1x2ml
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
