DAB is widely used for both immunoblotting and immunohistochemical staining techniques. It produces an insoluble end product that is brown in color and insoluble in alcohol. It is supplied as 50X concentrate.
DAB is the most common reagent employed for the immunohistochemical detection of horseradish peroxidase (HRP) labeled probes. DAB powder from different sources may show great variability with respect to purity. Histochemical grade material should be pale tan with no purple hue. The purple material is a DAB oxidation product that binds avidly to heme proteins such as myoglobin, hemoglobin and particularly, catalase. Therefore purple by-products should be avoided for histochemical uses.
United States Biological provides a stable DAB concentrate manufactured from carefully selected reagents. When diluted in appropriate buffers with added hydrogen peroxide, the resulting solution provides excellent results with most immunohistochemical and blotting procedures.
Note: DAB is a suspected carcinogen.
Color (Light tan to brown):
Passes
Stability (4 degrees C and RT):
Passes
Storage and Stability:
May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 6 months.
Related Products:
Tris HCl Buffer is supplied separately (Cat# D1000-05).
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