Stem Cells From Human Exfoliated Deciduous Teeth (SHED ) are considered to be the most 'immature' of the Dental Stem Cells and show the highest proliferative capacity when grown in culture. Similar to the DPSCs from third molars, SHED are very durable in culture and have been passaged over 80 times with no detectable signs of senescence or telomere shortening. SHED express the embryonic stem (ES) cell markers Oct4, Nanog, stage-specific embryonic antigens (SSEA-3, SSEA-4), and tumor recognition antigens (TRA-1-60 and TRA-1-81). When cultured in neurogenic medium, SHED form sphere-like clusters which adhere to the culture dish or float freely in the culture medium.
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