Dihydro-Testosterone BioAssay(TM) ELISA Kit

5a-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Intended Use: The Dihydro- Testosterone ELISA kis is for the direct quantitative determination of Dihydrotestosterone by enzyme immunoassay in human serum. For in vitro diagnostic use only. Principle of the Test: The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of DHT in the sample. A set of standards is used to plot a standard curve from which the amount of DHT in patient samples and controls can be directly read. Detection Range: 0-2500 pg/ml Specificity: 100% Sensitivity: 6pg/ml Kit Components: Microtiter Plate, 1x96wells Dihydrotestosterone-Horseradish Peroxidase (HRP) Conjugate Concentrate, 1x200ul Calibrator A 0pg/ml, 1x2ml Calibrator B 25pg/ml, 1x600ul Calibrator C 100pg/ml, 1x600ul Calibrator D 500pg/ml, 1x600ul Calibrator E 1000pg/ml, 1x600ul Calibrator F 2500pg/ml, 1x600ul Control, 1x600ul Wash Buffer Concentrate, 1x50ml Assay Buffer, 1x15ml TMB Substrate, 1x16ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4 degrees C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.