Source:
Recombinant protein corresponding to Human GMP, at N-terminal Ala expressed in E. coli.
Molecular Weight:
~16.5kD
Bioactivity:
GMP-grade Recombinant Human FGF basic stimulates proliferation of the NR6R-3T3 mouse fibro- blast cell line. The ED50 is typically 0.1-0.6ng/ml.
Endotoxin:
<0.10EU/1ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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