Ficin is used to digest formalin-fixed, paraffin-embedded tissues, and will improve the signal of certain antigens. It contains 50ml of ready-to-use ficin.
Simple Protocol:
1. Deparaffinize slides and hydrate to water.
2. Block endogenous peroxidase activity (if necessary).
3. Wash slides in several changes of distilled water.
4. Apply 2-4 drops to tissue sections.
5. Incubate for 5-10 minutes at 37 degrees C.*
6. Wash in several changes of PBS.
7. Resume immunostaining procedure.
* According to type of tissue and fixation...etc.
Storage and Stability:
Store at 4 degrees C. After use, return to 4 degrees C immediately.
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