A novel CHOK1/tGFP-BDKR2 cell line has been developed through stable transfection to modulate the internalization of the bradykinin receptor 2 (BDKR2) complex in living cells and to assay for compounds inducing tGFP-BDKR2 distribution inside the cells. BDKR2 Internalization Cell Line was obtained by transfection of an expression vector for a fusion protein of tGFP and human BDKR2, as well as G418 into CHOK1 cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-BDKR2 fusion protein. Assay Details: Innoprot BDKR2 Internalizacion Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate bradykinin receptor 2 internalization process following and quantifying the fluorescence distribution inside the cells. This highly reproducible assay allows monitoring BDKR2 receptor internalization process in High Content Analysis and fluorescence microscope applications.
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