A novel CHOK1/tGFP-CALCR cell line has been developed through stable transfection to modulate the internalization of the Calcitonin Receptor (CALCR) complex in living cells and to assay for compounds inducing tGFP-CALCR distribution inside the cells. Fluorescent CALCR Internalization Cell Line was obtained by transfection of an expression vector for a fusion protein of tGFP and human CALCR, as well as puromycin into CHOK1 cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-CALCR fusion protein.Assay Details: Innoprot CALCR Internalization Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate calcitonin receptor activation and the following redistribution process inside the cells. This highly reproducible assay allows monitoring CALCR activation and redistribution process in High Content Analysis and fluorescence microscope applications. ThIs internalization assay was validated with an average Z prime =0.60 +/- 0.02 for High Content Screening.
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