A novel CHOK1/tGFP-CB2 cell line has been developed through stable transfection to modulate the internalization of the cannabinoid receptor 2 (CB2, CNR2) complex in living cells and to assay for compounds inducing tGFP-CB2 distribution inside the cells. Fluorescent CB2 Internalization Assay Cell Line was obtained by stable transfection of an expression vector for a fusion protein of tGFP and human CB2, as well as G418 into CHOK1 cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-CB2 fusion protein. Assay Details: Innoprot CB2 Internalizacion Assay Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate cannabinoid receptor 2 internalization process following and quantifying the fluorescence distribution inside the cells. This highly reproducible assay allows monitoring CB2 receptor internalization process in High Content Analysis and fluorescence microscope applications.
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