A novel U2OS/tGFP-CCR2 cell line has been developed through stable transfection to modulate the internalization of the Human Chemokine (C-C motif) receptor 2 (CCR2) complex in living cells and to assay for compounds inducing tGFP-CCR2 redistribution inside the cells. Fluorescent CCR2 Internalization Assay cell line was obtained by stable transfection of an expression vector for a fusion protein of tGFP and human Chemokine (C-C motif) receptor 2, as well as G418 into U2OS cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-CCR2 fusion protein.Assay Details: Innoprot's CCR2 Internalization Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate Chemokine (C-C motif) receptor 2 activation and the following redistribution process inside the cells. This highly reproducible assay allows monitoring CCR2 receptor activation and redistribution process in High Content Analysis and fluorescence microscope applications. This internalization assay was validated with an average of Z=0.64 +/- 0.02 for High Content Screening.
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