A novel CHOK1/tGFP-CRHR2 cell line has been developed through stable transfection to modulate the internalization of the human corticotropin releasing hormone receptor 2 (CRHR2) complex in living cells and to assay for compounds inducing tGFP-CRHR2 distribution inside the cells.Fluorescent CRHR2 Internalization Assay Cell Line was obtained by transfection of an expression vector for a fusion protein of tGFP and human CRHR2, as well as Puromycin into CHOK1 cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-CRHR2 fusion protein.Assay Details:Innoprot CRHR2 Internalizacion Assay Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate human corticotropin releasing hormone receptor 2 internalization process following and quantifying the fluorescence distribution inside the cells.This highly reproducible assay allows monitoring CRHR2 receptor internalization process in High Content Analysis and fluorescence microscope applications.
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