Fucose Maleimide is designed for fucosylating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fucose Maleimide require free thiol groups. In the case of proteins, Fucose Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane). For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag, 5
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