Broad spectrum, cell culture antibiotic that is non toxic to viruses and mammalian cells at antibacterial levels. Inhibits growth of gram positive and negative bacteria, and mycoplasma. Inhibits normal protein synthesis by binding to L6 protein of 50S ribosomal subunit. Useful antibiotic for Baculovirus expression systems since mutations in rplF prevent Gentamycin from binding.
Appearance:
Colorless, clear solution
Solubility:
Colorless, clear, complete
Prepared from Gentamycin Sulfate USP:
Working Concentrations: 15-30ug/ml.
Potency: (same/more than) 590ug Gentamycin/mg
Specific Rotation:
+107 degrees to +121 degrees
Endotoxin: (same/less than)0.5EU/mg
Storage and Stability:
May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
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