Broad spectrum, cell culture antibiotic that is non toxic to viruses and mammalian cells at antibacterial levels. Inhibits growth of gram positive and negative bacteria, and mycoplasma. Inhibits normal protein synthesis by binding to L6 protein of 50S ribosomal subunit. Useful antibiotic for Baculovirus expression systems since mutations in rplF prevent Gentamycin from binding.
Prepared from
Gentamycin (Gentamicin) Sulfate
Prepared with ultrapure molecular biology grade water, 0.2um Sterile-filtered.
Potency:
(same/more than) 590ug
Gentamycin Content:
C1: 25-50%
C1a: 10-35%
C2a+C2: 25-55%
Working Concentrations: 15-30ug/ml.
Identity (IR):
Conforms to reference
Specific Rotation:
+107 degrees to +121 degrees
Endotoxin:
(same/less than)10EU/ml
Storage and Stability:
May be stored at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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