Glucagon BioAssay(TM) ELISA Kit (Human, Mouse, Rat)

Glucagon Enzyme Immunoassay (EIA) BioAssay(TM) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Glucagon antibody, both biotinylated Glucagon peptide and peptide standard or targeted peptide in samples interacts competitively with the Glucagon antibody. Uncompeted (bound) biotinylated Glucagon peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Glucagon peptide in the standard or samples. This is due to the competitive binding to Glucagon antibody between biotinylated Glucagon peptide and peptides in standard or samples. A standard curve of known concentration of Glucagon peptide can be established and the concentration of Glucagon peptide in the samples can be calculated accordingly. The minimum detectable concentration of Glucagon is 0.97pg/ml. Sample Type: Serum, plasma and cell culture media etc. Intended Use: The BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. Specificity: The capture antibody provided in this kit recognizes human, mouse and rat Glucagon. Sensitivity: The intra-assay reproducibility is CV<10% and inter-assay is CV<12%. Range: The detection range for the kit is 1-1000pg/ml Kit Components: Glucagon Microplate (Item A), 1x96wells Wash Buffer Concentrate (Item B), 1x25ml Lyophilized standard Glucagon peptide (Item C), 2x vials Lyophilized biotinylated Glucagon peptide (Item F), 2x vials HRP-Streptavidin concentrate (Item G), 1x600ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8ml Lyophilized positive control (Item M), 1x vial Lyophilized anti-Glucagon polyclonal antibody (Item N), 2x vials 1X Assay Diluent E (Item R), 2x vials Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.