Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of alpha-1,4 linkage with alpha-1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.
Sample Type:
Animal tissues: Liver, muscle, etc. Cell culture: Adherent or suspension cells.?
Intended Use:
The assay can detect less than 4ug/ml of Glycogen in various tissues. Analysis of metabolism and cell signaling.
Test Principle:
The Glycogen BioAssay(TM) kit II provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless Probe to a colored product with strong absorbance at 450nm.
Kit Components:
Glycogen Hydrolysis Buffer, 1x15ml
Glycogen Development Buffer, 1x25ml
Hydrolysis Enzyme Mix, lyophilized, 1x vial
Development Enzyme Mix, lyophilized, 1x vial
Probe, lyophilized, 1x vial
Glycogen Standard (2mg/ml), 1x100ul
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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