Green Fluorescent Protein (GFP) is used as a positive control when detecting GFP fusion proteins in GFP assays. Polyclonal GFP can detect GFP by ELISA (sandwich or capture) or western blot. Biotin conjugated polyclonal GFP used in a sandwich ELISA is well suited to titrate GFP in solution when used in combination with monoclonal GFP, using either form as the capture or detection. Control GFP can be used as a control in western blots. Researchers should determine optimal titers for applications.
Green Fluorescent Protein, expressed in E.coli.
Suitable for use as a control. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.