Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3a (51kD) and GSK-3b (46kD). GSK-3 has been implicated in the regulation of the c-Jun/AP-1 transcription factor and other intracellular signaling pathways.
Source:
Recombinant corresponding to human GSK-3b expressed in E.coli.
Molecular Weight:
~53-56kD
Biological Activity:
Activity of GSK-3b was determined by an in vitro kinase assay using 100ng Tau as substrate and 1ug of the recombinant GSK-3b in a 20ul reaction containing 25mM Tris-HCl, pH 7.5, 5mM b-glycerol phosphate, 12mM MgCl2, 2mM DTT, 0.1M Na3VO4, and 200uM ATP. After 30mins incubation at 30 degrees C, phosphorylation of Tau by the recombinant GSK-3b was determined by Western blot using a phospho-Tau antibody.
Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.