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HDAC2 Immunoprecipitation (IP) & Activity BioAssay(TM) Kit

Cat no: 167860


Supplier: United States Biological
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Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. These proteins have crucial roles in development and physiology, especially in the heart and nervous system. It is also deeply involved in cellular proliferation, cell cycle and apoptosis. Sample Type: Human, mouse or rat. Tissue extract, cell lysate and nuclear extract. Intended Use: Applications include: Immunoprecipitation of HDAC2 complex from cell, nuclear, or tissue extract. Measurement of HDAC2 activity of immunoprecipitated complex/purified enzyme and screening for activators or inhibitors of HDAC2. Test Principle: The HDAC2 IP & Activity BioAssay(TM) Kit provides an antibody-based method to specifically immunoprecipitate the HDAC2 complex from cells & tissues and to measure HDAC2 activity fluorometrically. HDAC2 is immunoprecipitated from cell or nuclear extract(s) using HDAC2 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader. Kit Components: HDAC Assay Buffer, 1x9ml Cell Lysis Buffer, 1x100ml HDAC Substrate, 1x100ul Developer, 1x500ul AFC (7-amino-4-trifluoromethyl coumarin) Standard, 1mM, 1x100ul Rabbit HDAC2 Antibody, 1x250ul Rabbit IgG (Control Antibody), 1x100ul Protein A/G Sepharose Beads (50% slurry in 20% ethanol/H2O), 1x650ul Positive Control (Jurkat Cell Lysate), lyophilized, 1x vial Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 167860
Applications: Immunoprecipitation
Size: 25Tests

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