Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.
Sample Type:
Testing candidate HDAC3 inhibitors
Intended Use:
Suitable for high throughput screening of HDAC3 inhibitors.
Kit Components:
HDAC3 assay buffer, 1x25ml
HDAC3 Substrate, 1x200ul
HDAC-3 enzyme, 1x200ul
Developer, 1x1ml
Trichostatin A, 1x50ul
Storage and Stability:
Store liquid at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.