Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In HDAC-3 activity Kit, HDAC-3 and Developer will deacetylate and cleave the substrate [R-H-K-K(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples.
Sample Type:
Cell and Tissue lysates, plasma and serum, other biological fluids.
Intended Use:
Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-3 samples.
Specificity:
Mammalian
Kit Components:
HDAC3 assay buffer, 1x25ml
HDAC3 substrate, 1x200ul
HDAC3 positive control, 1x20ul
AFC Standard (1mM), 1x100ul
Developer, 1x1ml
Trichostatin A, 1x200ul
Storage and Stability:
Store liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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