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His-tag (4C2) mAb

His-tag (4C2) mAb

Cat no: AP0032M

Supplier: Bioworld Technology, Inc.
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The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.
Catalogue number: AP0032M
Reactivities: Human, Mouse, Rat, Amoeba/Protozoa, Bacteria/Archaea, Bovine, C. elegans/Worm, Canine, Chemical, Chicken/Bird, Donkey, Drosophila/Arthropod, Epitope Tag, Feline, Goat, Guinea Pig, Hamster, Horse, Mollusc, Non-Human Primate, Plant, Porcine, Reptile, Rabbit
Hosts: Mouse
Applications: Western Blot
Size: 100ug/100ul
Swiss prot: N/A
Dilutions: WB:1:5000~10000
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide His-tag.
Species: His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6
Molecular weight: N/A
Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Additional info: For research use only, not for use in diagnostic procedure.

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