Factor X- gla domainless was prepared from purified Human Factor X using alphachymotrypsin. The alpha-chymotrypsin and all other products were removed using an anionexchange column after digestion. The above protein was purified from Human plasma thatwas tested and found negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II,HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA.Donors are screened for CJD (Creutzfeldt-Jakob Disease)