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IGF-BP1, Recombinant, Human, (IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1)

Cat no: 167877


Supplier: United States Biological
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Human IGF-BP1 is a 25.4kD cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a single, glycosylated protein consisting of 255aa residues with a N-terminal His-tag. Source: Recombinant corresponding to human IGF-BP1 expressed in Nso cells. Molecular Weight: ~28kD Biological Activity: The ED50 as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100ng/ml. Endotoxin: ~0.1ng/ul Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 167877
Size: 10ug
Form: Supplied as a lyophilized powder in 10mM HCl. Reconstitute with dH2O to 0.1-1.0mg/ml.
Purity: ~98% (HPLC, SDS-PAGE)

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