IGF-I (insulin-like Growth Factor-I) is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Murine IGF-I is a 7.6kD protein containing 70aa residues.
Source:
Recombinant corresponding to murine IGF-I expressed in E.coli.
Molecular Weight:
~8kD
Biological Activity:
The ED50 as determined by cell proliferation assay using FDC-P1 cells is ~2.0ng/ml, corresponding to a specific activity of ~5 x 10e5 units/mg. For most in vitro applications, the IGF-I exerts its biological activity in the concentration range of 1 to 20ng/ml.
Endotoxin:
~0.1ng/ul
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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