Immunoprecipitation (IP) is widely used in research and development, by which a protein can be selectively purified from samples.
Sample Type:
Biological samples and tissue or cell extracts.
Intended Use:
Used for Immunoprecipitation (IP) and Co-IP, SDS-PAGE or western blot analysis of Immunoprecipitated proteins/complexes and functional studies of Immunoprecipitated proteins/complexes.
Test Principle:
The immunoprecipitation BioAssay(TM) kit provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps. The protein A/G Sepharose beads provided in the kit has higher binding capacity with broader antibody isotype binding than traditional protein A or protein G resins. The kit can be used in variety of immunoprecipitation or Co-IP studies.
Kit Components:
Non-Denaturing Lysis Buffer, 1x40ml
RIPA Lysis Buffer, 1x40ml
Protease Inhibitor Cocktail, lyophilized, 1x vial
Wash Buffer, 1x20ml
Protein A/G Sepharose, 1x1ml
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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