Cayman's In Vitro Angiogenesis Assay uses a one-step model to study regulators of angiogenesis. Cell survival is improved compared to the other assays by use of a modified extracellular matrix that has been validated in both short-term (2-3 days) and long-term (up to ten days) experiments. Cayman's In Vitro Angiogenesis Assay includes PMA and JNJ-10198409 as controls for stimulation and inhibition of angiogenesis, respectively, as well as the fluorescent dye Calcein AM for visualization of cell organization.