Interleukin 15 (IL-15) Human, BioAssay(TM) ELISA Kit

IL-15 is a recently identified novel cytokine that shares many biological properties with IL-2, including T-Cell, B-Cell and NKcell-stimulatory activities. Source: Human Sample Type: Serum, plasma, cell culture supernatants and urine. Intended Use: The BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. This ELISA kit is specific for the measurement of human IL-15. The antibody pair provided in this kit recognizes human IL-15. Sensitivity: The intra-Assay reproducibility is CV <10% and inter-Assay is CV <12%. Test Principle: The Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) BioAssay(TM) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-15. This assay employs an antibody specific for human IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IL-15 is typically less than 3pg/ml. Kit Components: IL-15 Microplate (Item A), 1x96wells Wash Buffer Concentrate (Item B), 1x25ml Standards (Item C), 2x vials Assay Diluent A (Item D), 1x30ml Assay Diluent B (Item E), 1x15ml Detection Antibody IL-15 (Item F), 2x vials HRP-Streptavidin concentrate (Item G), 1x200ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8ml Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.