ISOLATE II RNA Micro Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from very small samples. Typical sample material comprises small numbers of cells (up to 5 x 105) and tissue (up to 5 mg) such as laser-captured cells, microdissected cryosections, fine needle aspirates, biopsy samples, pellets of cultured cells and flow cytometer sorted cells. The protocol is easy to follow on a step-by-step basis. The cells are lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases thus preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit). There is no need to perform a separate DNase I digestion step. The RNA is then bound to the silica membrane. The novel design allows elution of RNA in as little as 5
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