ISOLATE II RNA Mini Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of sources. The kit is compatible with animal tissues, cultured cells, bacterial cells, yeast and biological fluids (cell-free). Biological samples which are sometimes difficult to process will yield high-quality RNA i.e. mouse tissue (liver, brain), various tumour cell lines, Streptococcus and Actinobacillus pleuropneumoniae. The sample is lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases, preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit). There is no need to perform a separate DNase digestion step. The RNA is bound to the silica membrane, washed and pure, high quality RNA is eluted (Fig. 1). The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater
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