Canavanine, nitric oxide synthase inhibitor, is an analog of arginine. Both are imported into the cell via the same high-affinity permease, which is encoded by the can1 locus. High-level resistance to canavanine occurs exclusively because of mutation at this locus, but low-level resistance can arise at a number of other loci.
Because canavinine is a competitive inhibitor, arginine must be excluded from media used for testing sensitivity to the drug. Canavanine resistance must also be scored under high-nitrogen conditions, such as those provided by SD or SC medium, since the CAN1 permease will then provide the only entry route to the cell for arginine and canavanine. In the presence of low-nitrogen conditions, effectively those provided by YPD medium, the general amino acid permease (GAP) system is induced and arginine and canavanine can also be taken up by this route. In addition, Can r Arg-auxotrophs are viable on YPD but are inviable on synthetic media because they are unable to take up arginine.
Synonyms:
Canavalia ensiformis; O-Guanidino-L-homoserine, L-a-Amino-g-(guanidinooxy)-n-butyric acid sulfate
Purity (TLC):
(same/more than) 98%
Appearance:
White, crystalline powder
Solubility (100mg/ml, H2O): Colorless, clear, complete
Water (KF):
As reported
Elemental Analysis:
Carbon, Nitrogen: As reported
Stock Solution:
Prepare 100mg/ml in molecular biology grade dH2O. Filter sterilize (0.2um).
Working Solution:
60mg/L is typically used in autoclaved SD- or SC- arg medium for scoring and selecting canavanine resistance.
Storage:
Lyophilized powder may be stored at 4 degrees C.
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