Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.
Sample Type:
Cell and tissue culture supernatants, plasma and other biological fluids (optimized by end user).
Intended Use:
Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples. The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Test Principle:
Lipid Peroxidation BioAssay(TM) Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (y = 532nm) or fluorometrically (Ex/Em = 532/553nm).
Kit Components:
MDA Lysis Buffer, 1x25ml
Phosphotungstic Acid Solution, 1x12.5ml
BHT (100X), 1x1ml
TBA, 4x bottles
MDA Standard, 4.17M, 1x100ul
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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