IGF-I (insulin-like Growth Factor-I) is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Recombinant human IGF-I is a N-terminus modified IGF-I with a Long R3 chain, which shows higher potency than regular human IGF-I in bioactivity assay. The recombinant IGF-I Long R3 was produced from E. coli using Animal Origin Free (AOF) components and therefore it is suitable for bioproduction also. The product can be provided as lyophilized form or liquid form, stable for at least two years.
Source:
Recombinant corresponding to human Long R3 IGF-I expressed in E.coli.
Molecular Weight:
~9kD
Biological Activity:
The ED50 as determined by a cell proliferation assay using NIH3T3 cells is typically 1-6ng/ml.
Endotoxin:
~0.1ng/ul
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.