Home  >  Products  >  MAPKAPK3, Active, Recombinant, Human (Active MAPKAPK3, MAPKAPK3, MAPKAPK, human MAPKAPK3, active kinase, active kinases, recombinant MAPKAPK3, recombinant MAPKAPK3 kinase activity assay, protein kinase)
MAPKAPK3, Active, Recombinant, Human (Active MAPKAPK3, MAPKAPK3, MAPKAPK, human MAPKAPK3, active kinase, active kinases, recombinant MAPKAPK3, recombinant MAPKAPK3 kinase activity assay, protein kinase)

MAPKAPK3, Active, Recombinant, Human (Active MAPKAPK3, MAPKAPK3, MAPKAPK, human MAPKAPK3, active kinase, active kinases, recombinant MAPKAPK3, recombinant MAPKAPK3 kinase activity assay, protein kinase)

Cat no: 167909

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
MAPKAPK3 has a single potential SH3-binding site in the proline-rich N terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK2. MAPKAPK3 was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. MAPKAPK3 is uniquely poised to support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB. Source: Recombinant protein corresponding to human MAPKAPK3 expressed in Sf9 insect cells. Molecular Weight: ~69.0kD Biological Activity: 636nmol phosphate incorporated into MBP protein per minute per mg protein at 30 degrees C for 15 minutes using a final concentration of 50um ATP. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 167909
Size: 5ug
Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Purity: ~90% (SDS-PAGE)
References: 1. Sithanandam, G.; Latif, F.; Duh, F-M.; Bernal, R.; Smola, U.; Li, H.; Kuzmin, I.; Wixler, V.; Geil, L.; Shrestha, S.; Lloyd, P.; Bader, S.; Sekido, Y.; Tartof, K.D.; Kashuba, V.I.; Zabarovsky, E.R.; Dean, M.; Klein, G.; Lerman, M.I.; Minna, J.D.; Rapp, U.R.; Allikmets, R.: 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. Molec. Cell. Biol. 16: 868-876, 1996. 2. Ludwig, S.; Engel, K.; Hoffmeyer, A.; Sithanandam, G.; Neufeld, B.; Palm, D.; Gaestel, M.; Rapp, U.: 3pK, a novel mitogen-activated protein (MAP) kinase-activated protein kinase, is targeted by three MAP kinase pathways. Molec. Cell. Biol. 16: 6687-6697, 1996. 3. Neufeld B, Grosse-Wilde A, Hoffmeyer A, Jordan BW, Chen P, Dinev D, Ludwig S, Rapp UR. Serine/Threonine kinases 3pK and MAPK-activated protein kinase 2 interact with the basic helix-loop- helix transcription factor E47 and repress its transcriptional activity.J Biol Chem. 2000 Jul 7; 275(27):20239-42. 4. Maizels, E. T.; Mukherjee, A.; Sithanandam, G.; Peters, C. A.; Cottom, J.; Mayo, K. E.; Hunzicker- Dunn, M.: Developmental regulation of mitogen-activated protein kinase-activated kinases-2 and -3 (MAPKAPK-2/-3) in vivo during corpus luteum formation in the rat. Molec. Endocr. 15: 716-733, 2001.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave