Melanocortin-3 Receptor STEP Reporter Assay Kit (Luminescence)

Cat no: 600180

Supplier: Cayman Chemical Company

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Cayman's STEP Reporter Assays (Luminescence) consist of a 96-well plate pre-coated with a transfection complex consisting of the gene for the protein target of interest (i.e. an assay specific target protein), a SEAP reporter construct, and, in some cases, other genes needed to make the assay function with high fidelity. The plates are provide sealed and ready to use - all you need to do is add your cells. Transfection occurs overnight; cells can be tested the next day. The SEAP reporter is secreted into the media, so cellular responses can be followed over time. The kits are easily adapted to high throughput screening.

Catalogue number:	600180
Weight:	0
Form:	1 ea
P type:	Assay Kits\|STEP Assays
Shipping temp:	-20
Storage temp:	-20
Additional info:	The Melanocortin-3 receptor (MC3R) helps regulate energy homeostasis and mice lacking MC3R have increased fat mass and reduced lean mass. Therefore, agonists that selectively activate MC3R might have beneficial effects related to weight gain and glucose metabolism. This assay consists of a 96-well plate coated with both MC3R and Secretory Alkaline Phosphatase (SEAP) reporter constructs. Cells grown on the STEP complex will express MC3R at the cell surface. Binding of agonists to MC3R initiates a signaling cascade resulting in expression of SEAP. SEAP activity is measured following addition of luminescence-based alkaline phosphatase substrate provided in the kit.

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Melanocortin-3 Receptor STEP Reporter Assay Kit (Luminescence)

Cat no: 600180

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