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Myc-tag (5D10) mAb

Myc-tag (5D10) mAb

Cat no: AP0031M

Supplier: Bioworld Technology, Inc.
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A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
Catalogue number: AP0031M
Reactivities: Human, Mouse, Rat, Amoeba/Protozoa, Bacteria/Archaea, Bovine, C. elegans/Worm, Canine, Chemical, Chicken/Bird, Donkey, Drosophila/Arthropod, Epitope Tag, Feline, Goat, Guinea Pig, Hamster, Horse, Mollusc, Non-Human Primate, Plant, Porcine, Reptile, Rabbit
Hosts: Mouse
Applications: Western Blot
Size: 100ug/100ul
Swiss prot: N/A
Dilutions: WB:1:5000~10000
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide Myc-tag.
Species: The antibody detects over-expressed or recombinant proteins containing the Myc epitope tag.
Molecular weight: N/A
Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: Myc, c Myc Epitope Tag, c Myc Tag, Myc Epitope Tag, Myc proto-oncogene protein, Transcription factor p64,
Additional info: For research use only, not for use in diagnostic procedure.

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