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N-Acetylglucosamine, O-linked

N-Acetylglucosamine, O-linked

Cat no: A0528-19F

Supplier: United States Biological
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Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes (NPCs). NPCs contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IC): 1:100 Immunohistochemistry (Paraffin): 1:200 Immunohistochemistry: 1:10-1:200 Chromatin Immunoprecipitation: 1:10-1:500 Immunoprecipitation: 1:10-1:500 ELISA: 1:100-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0528-19F
Reactivities: Human, Mouse, Rat, Bovine, Drosophila/Arthropod, Xenopus/Amphibian
Hosts: Mouse
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified
References: 1. Han, I. et al. Interaction of the transcription factor Sp1 with the nuclear pore protein p62 requires the C-terminal domain of p62. J Cell Biochem 68: 50-61 (1998). 2. Sterne-Marr, R. et al. O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import. J Cell Biol 116: 271-280 (1992). 3. Holt, G.D. et al. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol 104: 1157-1164 (1987). 4. Snow, C.M. et al. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol 104: 1143-1156 (1987).
Additional info: Recognizes rat nuclear pore complex (NPC) O-linked glycoproteins. Species Crossreactivity: mouse, human, bovine, Drosophilia, fish, virus, Xenopus

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