The glycolytic enzyme enolase (2-phosph-D-glycerate hydrolyase) exists as several dimeric isoenzymes (aa, ab, ay and yy) composed of three distinct subunits, a, b, and y. Three isoenzymes are found in human brain: aa, ay and yy. The ay and yy-enolase isoenzymes are also known as neuron-specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. The NSE levels are low in health and benign subjects. Elevated levels are commonly found in patients with malignant tumors with neuronendocrine differentiation, especially small cell lung cancer and neuroblastoma. Lung cancer is one of the most spread cancer forms with incidences about 50~100 per 100,000 population. Approximately 20% of the lung cancer is small cell lung cancer. Patients with small cell lung cancer show various proportions of ay and yy isoenzyme. The determination of NSE should detect and y isoforms with the same sensitivity (1). The antibodies for this particular assay are specific for the y-subunit without cross reactivity with a or b subunits(1). NSE Kits are reported to be useful diagnostic marker for lung cancer (2), neuroblastoma (3), and melanoma (4), seminoma (S) and in injury of central nervous system (6). In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer.
Intended use:
The Neuron-Specific Enolase (NSE) enzyme linked immunosorbent assay (ELISA) provides quantitative measurement of human NSE in serum to aid in the clinical evaluation of patients suspected of having small cell lung cancer, and other related diseases.
cer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.
Test Principle
The NSE Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one monoclonal anti-gNSE antibody for solid phase (microtiter wells) immobilization and another monoclonal anti-gNSE antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The standards and test specimen (serum) are added to the antibody coated microtiter wells. During the incubation, specific NSE bound to anti-NSE antibody on the wells. Unbound NSE antigen is removed by washing the wells with buffer. Enzyme conjugate is then added to each well. After another incubation, unbound enzyme conjugate is washed off and the amount of bound peroxidase is proportional to the concentration of the NSE present in each sample. Upon addition of the substrate and chromogen, the intensity of blue color will develop in proportion to the concentration of NSE antigen in the samples.
Detection Range: 0-200ng/ml
Specificity: 98.7%
Sensitivity: 1.5ng/ml
Kit Components:
Microtiter Plate, 1x96wells
Sample DIluent, 1x12ml
Enzyme Conjugate Reagent, 1x12ml
Standard 0ng/ml, 1x1vial
Standard 5ng/ml, 1x1vial
Standard 15ng/ml, 1x1vial
Standard 40ng/ml, 1x1vial
Standard 100ng/ml, 1x1vial
Standard 200ng/ml, 1x1vial
Enzyme Conjugate Reagent, 1x12ml
TMB Substrate, 1x12ml
Stop Solution, 1x12ml
Storage and Stability:
Store all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.