Nuclear/Cytosol Extraction BioAssay(TM) Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations.
Sample Type:
Mitochondria, mitochondrial extract or cell lysate.
Intended Use:
This kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. Transcriptional activity/reporter assays/enzyme activity assays/Western blotting and more downstream assays.
Kit Components:
Cytosol Extraction Buffer A (CEB-A), 1x5ml
Cytosol Extraction Buffer B (CEB-B), 1x300ul
Nuclear Extraction Buffer A (NEB), 1x2.5ml
DTT, 1M, 1x100ul
Protease Inhibitor Cocktail, 1x vial
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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