Obestatin BioAssay(TM) ELISA Kit (Human, Mouse, Rat)

Obestatin Enzyme Immunoassay (EIA) BioAssay(TM) Kit is an in vitro quantitative assay for detecting Obestatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Obestatin antibody, both biotinylated Obestatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Obestatin antibody. Uncompeted (bound) biotinylated Obestatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Obestatin peptide in the standard or samples. This is due to the competitive binding to Obestatin antibody between biotinylated Obestatin peptide and peptides in standard or samples. A standard curve of known concentration of Obestatin peptide can be established and the concentration of Obestatin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Obestatin is 0.1 pg/ml. Sample Type: Serum, plasma and cell culture media etc. Intended Use: The BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. Specificity: The capture antibody provided in this kit recognizes human, mouse and rat Obestatin. This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC. Sensitivity: The intra-assay reproducibility is CV<10% and inter-assay is CV<12%. Range: The detection range for the kit is 0.1-1000pg/ml. Kit Components: Obestatin Microplate (Item A), 1x96wells Wash Buffer Concentrate (Item B), 1x25ml Standard Obestatin peptide (Item C), 2x vials Assay Diluent A (Item D), 1x30ml Assay Diluent B (Item E), 1x15 ml Biotinylated Obestatin peptide (Item F), 2x vials HRP-Streptavidin concentrate (Item G), 1x600ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8ml Positive control (Item M), 1x vial Anti- Obestatin polyclonal antibody (Item N), 2x vials Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.