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p-eIF4B(Ser422) pAb

p-eIF4B(Ser422) pAb

Cat no: AP0084


Supplier: Bioworld Technology, Inc.
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Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex
Catalogue number: AP0084
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug/100ul
Swiss prot: P23588
Dilutions: WB: 1:500~1:1000
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.
Species: p-eIF4B(Ser422) pAb detects endogenous levels of eIF4B protein only when phosphorylated at Ser422.
Molecular weight: ~ 80 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; Eukaryotic translation initiation factor 4B; PRO1843;
Additional info: For research use only, not for use in diagnostic procedure.

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