Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5kD heterodimer protein consisting of a 13.3kD A chain and a 12.2kD B chain.
Source:
Recombinant corresponding to human PDGF-AB expressed in E.coli.
Molecular Weight:
~26kD
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ~1ng/ml, corresponding to a specific activity of ~1x10e6units/mg.
Endotoxin:
~0.1ng/ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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