Recombinant mouse Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a homodimeric, non-glycosylated, polypeptide of 109 amino acids and having a molecular mass of 24.4kD.
Source:
Recombinant protein corresponding to mouse Platelet Derived Growth Factor expressed in E. coli.
Molecular Weight:
~24.4kD
Biological Activity:
The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 cells is < 2 ng/ml.
Endotoxin:
<0.1ng/ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.