Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-BB is a 24.3kD B chain homodimer protein formed by subunits of 109aa residues.
Source:
Recombinant corrosponding to Human PDGF-BB expressed in E.coli.
Molecular Weight:
~24kD
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of thimidine uptake by BALB/c 3T3 cells is ~1ng/ml, corresponding to a specific activity of ~1 x 10e6 units/mg.
Endotoxin:
~0.1ng/ug
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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