Phosphoglucomutase, Mammalian, BioAssay(TM) Kit (Colorimetric)

Phosphoglucomutase (PGM) plays a key role in carbohydrate metabolism and widely exists in all organisms. PGM interconverts Glucose-1-Phosphate (G1P) and Glucose-6-Phosphate (G6P) depending on the body requirement. When glycogen breaks down, G1P is generated and phosphoglucomutase converts G1P to G6P, which can go either to glycolytic pathway to generate ATP, or to pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, PGM converts G6P to G1P, which generates glycogen. In humans, phosphoglucomutase have 2 isoforms (PGM I and PGM II). PGM deficiency leads to glucose storage disease. Detection of abnormal phosphoglucomutase activity is crucial for diagnosis, prediction and treatment of the disease. In phosphoglucomutase assay, PGM converts glucose-1-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450nm. The Phosphoglucomutase Assay Kit is simple, sensitive and rapid and can detect PGM activity even less than 1mU/reaction. Sample Type: Animal tissues: Muscle, Liver, heart, kidney etc, cell culture, adherent or suspension cells, plasma Intended Use: Measurement of phosphoglucomutase activity in various tissues/cells. Analysis of glucose metabolism and cell signaling in various cell types. Screening anti-diabetic drugs. Sensitivity: <1mU/reaction Specificity: Mammalian Kit Components: PGM assay buffer, 1 x 27ml PGM substrate (lyophilized), 1 vial PGM Enzyme Mix (lyophilized), 1 vial PGM Developer (lyophilized), 1 vial NADH standard (lyophilized), 1 vial PGM positive control (lyophilized), 1 vial Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.