Phosphoglucose Isomerase, Mammalian, BioAssay(TM) Assay Kit (Colorimetric)

Phosphoglucose isomerase (PGI, EC 5.3.1.9) is an important housekeeping enzyme. PGI catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. PGI performs multiple functions & intracellularly plays key role in both glycolysis and gluconeogenesis. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as gastrointestinal, kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy. In the Phosphoglucose Isomerase Activity Assay Kit, PGI converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1mU/reaction. Sample Type: Animal tissues, cell culture, bacteria, yeast, etc Intended Use: Measurement of phosphoglucose isomerase activity in various tissues/cells, analysis of glucose metabolism and cell signaling in various cell types, screening anti-diabetic drugs. Sensitivity: <1mU/reaction Specificity: Mammalian Kit Components: PGI assay buffer, 1 x 27ml PGI substrate (lyophilized), 1 vial PGI enzyme mix (lyophilized) 1 vial PGI developer (lyophilized), 1 vial NADH standard (lyophilized), 1 vial PGI positive control (lyophilized), 1 vial Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.