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Products Prostaglandin D2 Express EIA Kit
| Catalogue number: | 512041 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Eicosanoids|Prostaglandins|Lipid Mediators|Lipids |
| Shipping temp: | -80 |
| Storage temp: | -80 |
| Additional info: | Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11(beta)-PGF2(alpha) and other metabolites. Measurement of the parent eicosanoid PGD2 is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman's PGD2 Express EIA Kit is a competitive assay that can be used for quantification of PGD2 in cell culture lysates and enzymatic reactions. This EIA offers the convenience of a fast assay with a two hour incubation time and one hour development time, while still achieving an IC50 value (50% B/B0) of approximately 1,300 pg/ml and a detection limit (80% B/B0) of approximately 350 pg/ml. |
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